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› 1i3i

X-ray diffraction
1.76Å resolution

Ribonuclease T1 V78T mutant

Released:
DOI: 10.2210/pdb1i3i/pdb
Model geometry
Fit model/data
PDB entry 1i3i coloured by chain, front view.
PDB entry 1i3i coloured by chain, side view.
PDB entry 1i3i coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Hydrophobic core manipulations in ribonuclease T1.
De Vos S, Backmann J, Prévost M, Steyaert J, Loris R
Biochemistry 40 10140-9 (2001)
PMID: 11513591

Function and Biology Details

Reaction catalysed: 
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment].
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.1 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 2GP 1 x 2GP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 40.523Å b: 46.872Å c: 50.234Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.202
Expression system: Escherichia coli

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Citations

10 citation in other articles

A mutation in the fibroblast growth factor 14 gene is associated with autosomal dominant cerebellar ataxia [corrected].
van Swieten et al. (2003)
9 more