› 1i3w

X-ray diffraction
1.7Å resolution

ACTINOMYCIN D BINDING TO CGATCGATCG

Released:
DOI: 10.2210/pdb1i3w/pdb
PDB entry 1i3w coloured by chain, front view.
PDB entry 1i3w coloured by chain, side view.
PDB entry 1i3w coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Crystallographic analysis of a novel complex of actinomycin D bound to the DNA decamer CGATCGATCG.
Robinson H, Gao YG, Yang X, Sanishvili R, Joachimiak A, Wang AH
Biochemistry 40 5587-92 (2001)
PMID: 11341823

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-113940 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ACTINOMYCIN D Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Streptomyces antibioticus
5'-D(*C*GP*AP*TP*CP*GP*AP*(BRU)P*CP*GP)-3' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
Source organism: Streptomyces antibioticus
Expression system: Not provided

Ligands and Environments

No bound ligands
4 modified residues:
Ligand BRU 4 x BRU
Ligand DVA 8 x DVA
Ligand SAR 8 x SAR
Ligand MVA 8 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 47.013Å b: 47.013Å c: 160.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 not available 0.279
Expression system: Not provided

Quick links

Citations

1 review citation

Unusual DNA duplex and hairpin motifs.
Chou et al. (2003)
16 other articles

2 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
1 more