› 1i4a

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF PHOSPHORYLATION-MIMICKING MUTANT T6D OF ANNEXIN IV

Released:
DOI: 10.2210/pdb1i4a/pdb
PDB entry 1i4a coloured by chain, front view.
PDB entry 1i4a coloured by chain, side view.
PDB entry 1i4a coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation.
Kaetzel MA, Mo YD, Mealy TR, Campos B, Bergsma-Schutter W, Brisson A, Dedman JR, Seaton BA
Biochemistry 40 4192-9 (2001)
PMID: 11300800

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-146508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Annexin A4 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 35.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13214 (Residues: 2-319; Coverage: 100%)
Gene names: ANX4, ANXA4
Sequence domains: Annexin
Structure domains: Annexin

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: R3
Unit cell:
a: 119.06Å b: 119.06Å c: 82.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Annexins: from structure to function.
Gerke et al. (2002)
10 more

2 mentions without citation

Conformational analysis of a genetically encoded FRET biosensor by SAXS.
Mertens et al. (2012)
1 more