Function and Biology Details
Reaction catalysed:
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Peptidase C14A, caspase catalytic domain
- Peptidase C14, caspase non-catalytic subunit p10
- Peptidase family C14A, cysteine active site
- Peptidase family C14A, His active site
- Peptidase C14, caspase domain
- Peptidase C14, p20 domain
- Caspase-like domain superfamily
- Baculovirus p35 superfamily
1 more domain
Structure domains:
Structure analysis Details
Assemblies composition:
Assembly name:
Early 35 kDa protein and Caspase-8 subunit p18 (preferred)
PDBe Complex ID:
PDB-CPX-139942 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Early 35 kDa protein
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.77 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Escherichia coli
UniProt:
Sequence domains: Apoptosis preventing protein
Structure domains: Baculovirus p35
Length: 299 amino acids
Theoretical weight: 34.77 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Escherichia coli
UniProt:
- Canonical:
P08160 (Residues: 2-299; Coverage: 100%)
Sequence domains: Apoptosis preventing protein
Structure domains: Baculovirus p35
Caspase-8 subunit p18
Molecule details ›
Chain: B
Length: 258 amino acids
Theoretical weight: 29.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Length: 258 amino acids
Theoretical weight: 29.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q14790 (Residues: 222-479; Coverage: 54%)
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 32-ID
Spacegroup:
C2221
Expression system: Escherichia coli
