Function and Biology Details
Reactions catalysed:
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157235 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Caspase-7 subunit p20
Molecule details ›
Chains: A, C
Length: 148 amino acids
Theoretical weight: 16.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Length: 148 amino acids
Theoretical weight: 16.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P55210 (Residues: 51-198; Coverage: 49%)
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Caspase-7 subunit p11
Molecule details ›
Chains: B, D
Length: 105 amino acids
Theoretical weight: 12.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Caspase domain
Structure domains: Caspase-like
Length: 105 amino acids
Theoretical weight: 12.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P55210 (Residues: 199-303; Coverage: 35%)
Sequence domains: Caspase domain
Structure domains: Caspase-like
E3 ubiquitin-protein ligase XIAP
Molecule details ›
Chains: E, F
Length: 117 amino acids
Theoretical weight: 13.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Inhibitor of Apoptosis domain
Length: 117 amino acids
Theoretical weight: 13.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P98170 (Residues: 124-240; Coverage: 24%)
Sequence domains: Inhibitor of Apoptosis domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
CHESS BEAMLINE A1
Spacegroup:
P3221
Expression system: Escherichia coli BL21(DE3)
