› 1i5i

X-ray diffraction
2.4Å resolution

THE C18S MUTANT OF BOVINE (GAMMA-B)-CRYSTALLIN

Released:
DOI: 10.2210/pdb1i5i/pdb
PDB entry 1i5i coloured by chain, front view.
PDB entry 1i5i coloured by chain, side view.
PDB entry 1i5i coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Enhanced crystallization of the Cys18 to Ser mutant of bovine gammaB crystallin.
Asherie N, Pande J, Pande A, Zarutskie JA, Lomakin J, Lomakin A, Ogun O, Stern LJ, King J, Benedek GB
J Mol Biol 314 663-9 (2001)
PMID: 11733987

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin B Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.98 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02526 (Residues: 2-175; Coverage: 99%)
Gene name: CRYGB
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 57.85Å b: 57.85Å c: 98.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.243
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The two-step mechanism of nucleation of crystals in solution.
Vekilov PG. (2010)
2 more

1 mention without citation

Aggregation of deamidated human betaB2-crystallin and incomplete rescue by alpha-crystallin chaperone.
Michiel et al. (2010)