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› 1i5s

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE KIF1A MOTOR DOMAIN COMPLEXED WITH MG-ADP

Released:
DOI: 10.2210/pdb1i5s/pdb
Model geometry
Fit model/data
PDB entry 1i5s coloured by chain, front view.
PDB entry 1i5s coloured by chain, side view.
PDB entry 1i5s coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Switch-based mechanism of kinesin motors.
Kikkawa M, Sablin EP, Okada Y, Yajima H, Fletterick RJ, Hirokawa N
Nature 411 439-45 (2001)
PMID: 11373668

Function and Biology Details

Reaction catalysed: 
ATP + H2O + a kinesin associated with a microtubule at position (n) =ADP + phosphate a kinesin associated with a microtubule at position (n+1,toward the plus end).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF1A Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 41.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33173 (Residues: 1-361; Coverage: 21%)
Gene names: Atsv, Kif1, Kif1a
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 41.672Å b: 51.925Å c: 157.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Intracellular transport and kinesin superfamily proteins, KIFs: structure, function, and dynamics.
Hirokawa et al. (2008)
26 more

21 mentions without citation

A comparative study of motor-protein motions by using a simple elastic-network model.
Zheng et al. (2003)
20 more