› 1i60

X-ray diffraction
1.6Å resolution

Structural genomics, IOLI protein

Released:
DOI: 10.2210/pdb1i60/pdb
PDB entry 1i60 coloured by chain, front view.
PDB entry 1i60 coloured by chain, side view.
PDB entry 1i60 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of Bacillus subtilis ioli shows endonuclase IV fold with altered Zn binding.
Zhang RG, Dementieva I, Duke N, Collart F, Quaite-Randall E, Alkire R, Dieckman L, Maltsev N, Korolev O, Joachimiak A
Proteins 48 423-6 (2002)
PMID: 12112707

Function and Biology Details

Reaction catalysed: 
2,4,6/3,5-pentahydroxycyclohexanone = 2D-2,3,5/4,6-pentahydroxycyclohexanone
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosose isomerase Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.98 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42419 (Residues: 1-278; Coverage: 100%)
Gene names: B65B, BSU39680, iolI, yxdH
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 74.291Å b: 104.771Å c: 48.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.228
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Automation of protein purification for structural genomics.
Kim et al. (2004)
9 more

2 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
1 more