› 1i80

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF M. TUBERCULOSIS PNP IN COMPLEX WITH IMINORIBITOL, 9-DEAZAHYPOXANTHINE AND PHOSPHATE ION

Released:
DOI: 10.2210/pdb1i80/pdb
PDB entry 1i80 coloured by chain, front view.
PDB entry 1i80 coloured by chain, side view.
PDB entry 1i80 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structures of purine nucleoside phosphorylase from Mycobacterium tuberculosis in complexes with immucillin-H and its pieces.
Shi W, Basso LA, Santos DS, Tyler PC, Furneaux RH, Blanchard JS, Almo SC, Schramm VL
Biochemistry 40 8204-15 (2001)
PMID: 11444966

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 268 amino acids
Theoretical weight: 27.6 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WP01 (Residues: 1-268; Coverage: 100%)
Gene names: MTV016.06, Rv3307, deoD, punA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand 9HX 3 x 9HX
Ligand IMR 1 x IMR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21212
Unit cell:
a: 119.984Å b: 135.773Å c: 45.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Structural analyses reveal two distinct families of nucleoside phosphorylases.
Pugmire et al. (2002)
4 more