› 1i8f

X-ray diffraction
1.75Å resolution

THE CRYSTAL STRUCTURE OF A HEPTAMERIC ARCHAEAL SM PROTEIN: IMPLICATIONS FOR THE EUKARYOTIC SNRNP CORE

Released:
DOI: 10.2210/pdb1i8f/pdb
PDB entry 1i8f coloured by chain, front view.
PDB entry 1i8f coloured by chain, side view.
PDB entry 1i8f coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
The crystal structure of a heptameric archaeal Sm protein: Implications for the eukaryotic snRNP core.
Mura C, Cascio D, Sawaya MR, Eisenberg DS
Proc Natl Acad Sci U S A 98 5532-7 (2001)
PMID: 11331747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative snRNP Sm-like protein Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 81 amino acids
Theoretical weight: 8.94 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZYG5 (Residues: 1-80; Coverage: 100%)
Gene name: PAE0790
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2
Unit cell:
a: 100.261Å b: 95.738Å c: 62.157Å
α: 90° β: 92.69° γ: 90°
R-values:
R R work R free
0.235 0.235 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

LSm proteins form heptameric rings that bind to RNA via repeating motifs.
Khusial et al. (2005)
11 more

10 mentions without citation

In vitro assembly of Haemophilus influenzae adhesin transmembrane domain and studies on the electrostatic repulsion at the interface.
Aoki et al. (2019)
9 more