› 1i9a

X-ray diffraction
2.5Å resolution

STRUCTURAL STUDIES OF CHOLESTEROL BIOSYNTHESIS: MEVALONATE 5-DIPHOSPHATE DECARBOXYLASE AND ISOPENTENYL DIPHOSPHATE ISOMERASE

Released:
DOI: 10.2210/pdb1i9a/pdb
PDB entry 1i9a coloured by chain, front view.
PDB entry 1i9a coloured by chain, side view.
PDB entry 1i9a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis.
Bonanno JB, Edo C, Eswar N, Pieper U, Romanowski MJ, Ilyin V, Gerchman SE, Kycia H, Studier FW, Sali A, Burley SK
Proc Natl Acad Sci U S A 98 12896-901 (2001)
PMID: 11698677

Function and Biology Details

Reaction catalysed: 
Isopentenyl diphosphate = prenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl-diphosphate Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46822 (Residues: 1-182; Coverage: 100%)
Gene names: JW2857, b2889, idi, ygfV
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P41212
Unit cell:
a: 72.758Å b: 72.758Å c: 204.471Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.279
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
9 more

4 mentions without citation

Structural genomics: a pipeline for providing structures for the biologist.
Chance et al. (2002)
3 more