› 1i9h

X-ray diffraction
2.4Å resolution

CORE STREPTAVIDIN-BNA COMPLEX

Released:
DOI: 10.2210/pdb1i9h/pdb
PDB entry 1i9h coloured by chain, front view.
PDB entry 1i9h coloured by chain, side view.
PDB entry 1i9h coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Chicken avidin exhibits pseudo-catalytic properties. Biochemical, structural, and electrostatic consequences.
Huberman T, Eisenberg-Domovich Y, Gitlin G, Kulik T, Bayer EA, Wilchek M, Livnah O
J Biol Chem 276 32031-9 (2001)
PMID: 11395489

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 14.5 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 25-163; Coverage: 87%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand BNI 2 x BNI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 47.121Å b: 95.16Å c: 105.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.177 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
2 more

1 mention without citation

Structural elements responsible for conversion of streptavidin to a pseudoenzyme.
Eisenberg-Domovich et al. (2004)