› 1i9v

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX

Released:
DOI: 10.2210/pdb1i9v/pdb
PDB entry 1i9v coloured by chain, front view.
PDB entry 1i9v coloured by chain, side view.
PDB entry 1i9v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin B complex.
Mikkelsen NE, Johansson K, Virtanen A, Kirsebom LA
Nat Struct Biol 8 510-4 (2001)
PMID: 11373618

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195823 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
PHENYLALANINE TRANSFER RNA Chain: A
Molecule details ›
Chain: A
Length: 76 nucleotides
Theoretical weight: 24.76 KDa
Source organism: Saccharomyces cerevisiae
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand NMY 1 x NMY
Ligand MG 3 x MG
2 modified residues:
Ligand YG 1 x YG
Ligand 5MU 1 x 5MU

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 54.9Å b: 32.9Å c: 63.1Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.23 0.228 0.275

Quick links

Citations

11 review citations

Targeting RNA with small molecules.
Tor Y. (2003)
10 more

10 mentions without citation

Drugging tRNA aminoacylation.
Ho et al. (2018)
9 more