Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
Type II restriction enzyme NaeI and DNA (preferred)
PDBe Complex ID:
PDB-CPX-113949 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme NaeI
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.38 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli
UniProt:
Sequence domains: Restriction endonuclease NaeI
Structure domains:
Length: 317 amino acids
Theoretical weight: 35.38 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli
UniProt:
- Canonical:
P50187 (Residues: 1-317; Coverage: 100%)
Sequence domains: Restriction endonuclease NaeI
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X12C
Spacegroup:
P212121
Expression systems:
- Escherichia coli
- Not provided
