› 1iay

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF ACC SYNTHASE COMPLEXED WITH COFACTOR PLP AND INHIBITOR AVG

Released:
DOI: 10.2210/pdb1iay/pdb
PDB entry 1iay coloured by chain, front view.
PDB entry 1iay coloured by chain, side view.
PDB entry 1iay coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
Crystal structures of 1-aminocyclopropane-1-carboxylate (ACC) synthase in complex with aminoethoxyvinylglycine and pyridoxal-5'-phosphate provide new insight into catalytic mechanisms.
Huai Q, Xia Y, Chen Y, Callahan B, Li N, Ke H
J Biol Chem 276 38210-6 (2001)
PMID: 11431475

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148386 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 48.29 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18485 (Residues: 11-438; Coverage: 88%)
Gene names: ACC2, ACS2, PCVV4A
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand AVG 1 x AVG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6222
Unit cell:
a: 123.1Å b: 123.1Å c: 106.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.29
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Eto Brute? Role of ACS turnover in regulating ethylene biosynthesis.
Chae et al. (2005)
2 more

4 mentions without citation

A conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species.
Boualem et al. (2009)
3 more