› 1ib0

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD AND NAD

Released:
DOI: 10.2210/pdb1ib0/pdb
PDB entry 1ib0 coloured by chain, front view.
PDB entry 1ib0 coloured by chain, side view.
PDB entry 1ib0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent.
Bewley MC, Marohnic CC, Barber MJ
Biochemistry 40 13574-82 (2001)
PMID: 11695905

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P20070 (Residues: 34-301; Coverage: 89%)
Gene names: Cyb5r3, Dia1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P212121
Unit cell:
a: 67.612Å b: 69.458Å c: 78.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Recessive congenital methaemoglobinaemia: cytochrome b(5) reductase deficiency.
Percy et al. (2008)
8 more

6 mentions without citation

Cytochrome b5 reductases: Redox regulators of cell homeostasis.
Hall et al. (2022)
5 more