› 1ic7

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF HYHEL-10 FV MUTANT(HD32A99A)-HEN LYSOZYME COMPLEX

Released:
DOI: 10.2210/pdb1ic7/pdb
PDB entry 1ic7 coloured by chain, front view.
PDB entry 1ic7 coloured by chain, side view.
PDB entry 1ic7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction: the case of hen lysozyme-HyHEL-10 Fv complex.
Shiroishi M, Yokota A, Tsumoto K, Kondo H, Nishimiya Y, Horii K, Matsushima M, Ogasahara K, Yutani K, Kumagai I
J Biol Chem 276 23042-50 (2001)
PMID: 11297547

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132836 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig kappa chain V-V region L7 Chain: L
Molecule details ›
Chain: L
Length: 107 amino acids
Theoretical weight: 11.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01642 (Residues: 21-115; Coverage: 100%)
Gene name: Gm10881
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain V region 36-60 Chain: H
Molecule details ›
Chain: H
Length: 114 amino acids
Theoretical weight: 12.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01823 (Residues: 1-113; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Lysozyme C Chain: Y
Molecule details ›
Chain: Y
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P41212
Unit cell:
a: 57.215Å b: 57.215Å c: 237.903Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.177 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Quinary protein structure and the consequences of crowding in living cells: leaving the test-tube behind.
Wirth et al. (2013)
1 more

2 mentions without citation

RosettaAntibodyDesign (RAbD): A general framework for computational antibody design.
Adolf-Bryfogle et al. (2018)
1 more