› 1icq

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF 12-OXOPHYTODIENOATE REDUCTASE 1 FROM TOMATO COMPLEXED WITH 9R,13R-OPDA

Released:
DOI: 10.2210/pdb1icq/pdb
PDB entry 1icq coloured by chain, front view.
PDB entry 1icq coloured by chain, side view.
PDB entry 1icq coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.
Breithaupt C, Strassner J, Breitinger U, Huber R, Macheroux P, Schaller A, Clausen T
Structure 9 419-29 (2001)
PMID: 11377202

Function and Biology Details

Reaction catalysed: 
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 42.44 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9XG54 (Residues: 1-376; Coverage: 100%)
Gene name: OPR1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand OPD 2 x OPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P1
Unit cell:
a: 53.583Å b: 72.759Å c: 72.616Å
α: 62.42° β: 85.19° γ: 79.96°
R-values:
R R work R free
0.198 0.198 0.234
Expression system: Escherichia coli BL21

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Citations

7 review citations

Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany.
Wasternack et al. (2013)
6 more