› 1idr

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE TRUNCATED-HEMOGLOBIN-N FROM MYCOBACTERIUM TUBERCULOSIS

Released:
DOI: 10.2210/pdb1idr/pdb
PDB entry 1idr coloured by chain, front view.
PDB entry 1idr coloured by chain, side view.
PDB entry 1idr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mycobacterium tuberculosis hemoglobin N displays a protein tunnel suited for O2 diffusion to the heme.
Milani M, Pesce A, Ouellet Y, Ascenzi P, Guertin M, Bolognesi M
EMBO J 20 3902-9 (2001)
PMID: 11483493

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 1 truncated hemoglobin GlbN Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 14.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN25 (Residues: 1-136; Coverage: 100%)
Gene names: MTCY48.23, Rv1542c, glbN
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 44.8Å b: 62.248Å c: 91.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.25
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Acyl-CoA dehydrogenases. A mechanistic overview.
Ghisla et al. (2004)
15 more

17 mentions without citation

Mycobacterium tuberculosis hemoglobin N displays a protein tunnel suited for O2 diffusion to the heme.
Milani et al. (2001)
16 more