1ie0

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF LUXS

Released:
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal site.
Proc Natl Acad Sci U S A 98 11169-74 (2001)
PMID: 11553770

Function and Biology Details

Reaction catalysed:
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-128577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-ribosylhomocysteine lyase Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.79 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34667 (Residues: 1-157; Coverage: 100%)
Gene names: BSU30670, luxS, ytjB
Sequence domains: S-Ribosylhomocysteinase (LuxS)
Structure domains: S-ribosylhomocysteinase (LuxS)

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6522
Unit cell:
a: 62.732Å b: 62.732Å c: 150.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.209
Expression system: Escherichia coli BL21(DE3)