› 1ie3

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF R153C E. COLI MALATE DEHYDROGENASE

Released:
DOI: 10.2210/pdb1ie3/pdb
PDB entry 1ie3 coloured by chain, front view.
PDB entry 1ie3 coloured by chain, side view.
PDB entry 1ie3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analyses of a malate dehydrogenase with a variable active site.
Bell JK, Yennawar HP, Wright SK, Thompson JR, Viola RE, Banaszak LJ
J Biol Chem 276 31156-62 (2001)
PMID: 11389141

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 312 amino acids
Theoretical weight: 32.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P61889 (Residues: 1-312; Coverage: 100%)
Gene names: JW3205, b3236, mdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand PYR 1 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 147.41Å b: 52.85Å c: 169.91Å
α: 90° β: 101.76° γ: 90°
R-values:
R R work R free
0.186 0.186 0.252
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular recognition of oxoanions based on guanidinium receptors.
Blondeau et al. (2007)
1 more

3 mentions without citation

Structure of Escherichia coli malate dehydrogenase at 1.45 A resolution.
Zaitseva et al. (2009)
2 more