› 1igw

X-ray diffraction
2.1Å resolution

Crystal Structure of the Isocitrate Lyase from the A219C mutant of Escherichia coli

Released:
DOI: 10.2210/pdb1igw/pdb
PDB entry 1igw coloured by chain, front view.
PDB entry 1igw coloured by chain, side view.
PDB entry 1igw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure and domain organization of Escherichia coli isocitrate lyase.
Britton KL, Abeysinghe IS, Baker PJ, Barynin V, Diehl P, Langridge SJ, McFadden BA, Sedelnikova SE, Stillman TJ, Weeradechapon K, Rice DW
Acta Crystallogr D Biol Crystallogr 57 1209-18 (2001)
PMID: 11526312

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 434 amino acids
Theoretical weight: 47.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9G6 (Residues: 1-434; Coverage: 100%)
Gene names: JW3975, aceA, b4015, icl
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

3 bound ligands:
Ligand HG 21 x HG
Ligand MG 4 x MG
Ligand PYR 4 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P32
Unit cell:
a: 88.65Å b: 88.65Å c: 199.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis.
Dunn et al. (2009)
2 more

8 mentions without citation

Potential inhibitors for isocitrate lyase of Mycobacterium tuberculosis and non-M. tuberculosis: a summary.
Lee et al. (2015)
7 more