› 1ih7

X-ray diffraction
2.21Å resolution

High-Resolution Structure of Apo RB69 DNA Polymerase

Released:
DOI: 10.2210/pdb1ih7/pdb
PDB entry 1ih7 coloured by chain, front view.
PDB entry 1ih7 coloured by chain, side view.
PDB entry 1ih7 coloured by chain, top view.
Source organism: Escherichia phage RB69
Primary publication:
Structure of the replicating complex of a pol alpha family DNA polymerase.
Franklin MC, Wang J, Steitz TA
Cell 105 657-67 (2001)
PMID: 11389835

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 903 amino acids
Theoretical weight: 104.74 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand GMP 1 x GMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 80.196Å b: 116.309Å c: 194.391Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

60 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
59 more

19 mentions without citation

Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives.
Mueser et al. (2010)
18 more