› 1iha

X-ray diffraction
1.6Å resolution

Structure of the Hybrid RNA/DNA R-GCUUCGGC-D[BR]U in Presence of RH(NH3)6+++

Released:
DOI: 10.2210/pdb1iha/pdb
PDB entry 1iha coloured by chain, front view.
PDB entry 1iha coloured by chain, side view.
PDB entry 1iha coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Destabilizing effect of a fluorouracil extra base in a hybrid RNA duplex compared with bromo and chloro analogues.
Cruse W, Saludjian P, Neuman A, Prangé T
Acta Crystallogr D Biol Crystallogr 57 1609-13 (2001)
PMID: 11679725

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113079 (preferred)
Entry contents:
1 distinct DNA/RNA hybrid molecule
Macromolecule:
5'-R(*GP*CP*UP*UP*CP*GP*GP*C)-D(P*(BRU))-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 9 nucleotides
Theoretical weight: 2.89 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand RHD 2 x RHD
Ligand CL 2 x CL
1 modified residue:
Ligand BRU 2 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: C2
Unit cell:
a: 53.8Å b: 19.4Å c: 50.31Å
α: 90° β: 109.9° γ: 90°
R-values:
R R work R free
0.167 0.162 0.196
Expression system: Not provided

Quick links

Citations

3 citation in other articles

A general strategy to solve the phase problem in RNA crystallography.
Keel et al. (2007)
2 more

2 mentions without citation

How far are we from automatic crystal structure solution via molecular-replacement techniques?
Burla et al. (2020)
1 more