1ihf

X-ray diffraction
2.5Å resolution

INTEGRATION HOST FACTOR/DNA COMPLEX

Released:
Source organism: Escherichia coli

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-113966 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Integration host factor subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.37 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A6X7 (Residues: 1-99; Coverage: 100%)
Gene names: JW1702, b1712, hid, himA, ihfA
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
Integration host factor subunit beta Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A6Y1 (Residues: 1-94; Coverage: 100%)
Gene names: JW0895, b0912, himD, hip, ihfB
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
DNA (35-MER) Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.8 KDa
DNA (5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
DNA (5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*C P*AP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.07 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 47.36Å b: 59.92Å c: 180.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.192 0.255