› 1ii6

X-ray diffraction
2.1Å resolution

Crystal Structure of the Mitotic Kinesin Eg5 in Complex with Mg-ADP.

Released:
DOI: 10.2210/pdb1ii6/pdb
PDB entry 1ii6 coloured by chain, front view.
PDB entry 1ii6 coloured by chain, side view.
PDB entry 1ii6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker.
Turner J, Anderson R, Guo J, Beraud C, Fletterick R, Sakowicz R
J Biol Chem 276 25496-502 (2001)
PMID: 11328809

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52732 (Residues: 1-368; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand NO3 2 x NO3
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 53.083Å b: 78.588Å c: 94.147Å
α: 90° β: 93.84° γ: 90°
R-values:
R R work R free
0.218 0.218 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Kinesins and cancer.
Rath et al. (2012)
19 more

29 mentions without citation

Force generation by kinesin and myosin cytoskeletal motor proteins.
Kull et al. (2013)
28 more