› 1ii7

X-ray diffraction
2.2Å resolution

Crystal structure of P. furiosus Mre11 with manganese and dAMP

Released:
DOI: 10.2210/pdb1ii7/pdb
PDB entry 1ii7 coloured by chain, front view.
PDB entry 1ii7 coloured by chain, side view.
PDB entry 1ii7 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural biochemistry and interaction architecture of the DNA double-strand break repair Mre11 nuclease and Rad50-ATPase.
Hopfner KP, Karcher A, Craig L, Woo TT, Carney JP, Tainer JA
Cell 105 473-85 (2001)
PMID: 11371344

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA double-strand break repair protein Mre11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 38.93 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1N9 (Residues: 1-333; Coverage: 78%)
Gene names: PF1166, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 4 x MN
Ligand SO4 3 x SO4
Ligand DA 1 x DA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 74.29Å b: 88.74Å c: 145.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.264
Expression system: Escherichia coli

Quick links

Citations

85 review citations

Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair.
Symington LS. (2002)
84 more

22 mentions without citation

Structural studies of DNA end detection and resection in homologous recombination.
Schiller et al. (2014)
21 more