› 1ij1

X-ray diffraction
1.86Å resolution

GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position

Released:
DOI: 10.2210/pdb1ij1/pdb
PDB entry 1ij1 coloured by chain, front view.
PDB entry 1ij1 coloured by chain, side view.
PDB entry 1ij1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Buried polar residues in coiled-coil interfaces.
Akey DL, Malashkevich VN, Kim PS
Biochemistry 40 6352-60 (2001)
PMID: 11371197

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 34 amino acids
Theoretical weight: 4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 25.683Å b: 42.192Å c: 42.682Å
α: 90° β: 98.87° γ: 90°
R-values:
R R work R free
0.232 0.227 0.278
Expression system: Not provided

Quick links

Citations

6 review citations

Protein oligomerization: how and why.
Ali et al. (2005)
5 more

1 mention without citation

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)