› 1ij3

X-ray diffraction
1.8Å resolution

GCN4-pVSL Coiled-coil trimer with Serine at the a(16) position

Released:
DOI: 10.2210/pdb1ij3/pdb
PDB entry 1ij3 coloured by chain, front view.
PDB entry 1ij3 coloured by chain, side view.
PDB entry 1ij3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Buried polar residues in coiled-coil interfaces.
Akey DL, Malashkevich VN, Kim PS
Biochemistry 40 6352-60 (2001)
PMID: 11371197

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 34 amino acids
Theoretical weight: 4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 25.564Å b: 25.839Å c: 34.373Å
α: 86.47° β: 84.49° γ: 79.4°
R-values:
R R work R free
0.22 0.22 0.269
Expression system: Not provided

Quick links

Citations

7 review citations

Protein oligomerization: how and why.
Ali et al. (2005)
6 more

3 mentions without citation

A conserved trimerization motif controls the topology of short coiled coils.
Kammerer et al. (2005)
2 more