› 1ij6

X-ray diffraction
3.1Å resolution

CA2+-BOUND STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD

Released:
DOI: 10.2210/pdb1ij6/pdb
PDB entry 1ij6 coloured by chain, front view.
PDB entry 1ij6 coloured by chain, side view.
PDB entry 1ij6 coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum.
Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M
Structure 11 75-85 (2003)
PMID: 12517342

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmodial-specific protein LAV1-2 Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 37.14 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: P14725 (Residues: 33-355; Coverage: 91%)
Sequence domains: EF hand
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 64.4Å b: 64.4Å c: 209.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.293
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Two structural motifs within canonical EF-hand calcium-binding domains identify five different classes of calcium buffers and sensors.
Denessiouk et al. (2014)
2 more

1 mention without citation

Testing water-mediated DNA recognition by the Hin recombinase.
Chiu et al. (2002)