› 1im4

X-ray diffraction
2.3Å resolution

Crystal Structure of a DinB Homolog (DBH) Lesion Bypass DNA Polymerase Catalytic Fragment from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb1im4/pdb
PDB entry 1im4 coloured by chain, front view.
PDB entry 1im4 coloured by chain, side view.
PDB entry 1im4 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of a DinB lesion bypass DNA polymerase catalytic fragment reveals a classic polymerase catalytic domain.
Zhou BL, Pata JD, Steitz TA
Mol Cell 8 427-37 (2001)
PMID: 11545744

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase IV Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.84 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P96022 (Residues: 2-216; Coverage: 61%)
Gene name: dbh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 85.988Å b: 87.542Å c: 76.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.261
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
21 more

2 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
1 more