› 1im8

X-ray diffraction
2.2Å resolution

Crystal structure of YecO from Haemophilus influenzae (HI0319), a methyltransferase with a bound S-adenosylhomocysteine

Released:
DOI: 10.2210/pdb1im8/pdb
PDB entry 1im8 coloured by chain, front view.
PDB entry 1im8 coloured by chain, side view.
PDB entry 1im8 coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine.
Lim K, Zhang H, Tempczyk A, Bonander N, Toedt J, Howard A, Eisenstein E, Herzberg O
Proteins 45 397-407 (2001)
PMID: 11746687

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-S-adenosyl-L-methionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 28.02 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P43985 (Residues: 1-241; Coverage: 100%)
Gene names: HI_0319, cmoA
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SAI 2 x SAI
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6122
Unit cell:
a: 74.97Å b: 74.97Å c: 319.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.255
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Overview of structural genomics: from structure to function.
Zhang et al. (2003)
17 other articles

13 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
12 more