› 1inl

X-ray diffraction
1.5Å resolution

Crystal Structure of Spermidine Synthase from Thermotoga Maritima

Released:
DOI: 10.2210/pdb1inl/pdb
PDB entry 1inl coloured by chain, front view.
PDB entry 1inl coloured by chain, side view.
PDB entry 1inl coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor.
Korolev S, Ikeguchi Y, Skarina T, Beasley S, Arrowsmith C, Edwards A, Joachimiak A, Pegg AE, Savchenko A
Nat Struct Biol 9 27-31 (2002)
PMID: 11731804

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 296 amino acids
Theoretical weight: 34.18 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZC2 (Residues: 1-296; Coverage: 100%)
Gene names: TM_0654, speE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 132.436Å b: 197.812Å c: 51.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.213
Expression system: Escherichia coli

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Citations

13 review citations

Polyamines: essential factors for growth and survival.
Kusano et al. (2008)
12 more

7 mentions without citation

Alda-1 is an agonist and chemical chaperone for the common human aldehyde dehydrogenase 2 variant.
Perez-Miller et al. (2010)
6 more