› 1iqq

X-ray diffraction
1.5Å resolution

Crystal Structure of Japanese pear S3-RNase

Released:
DOI: 10.2210/pdb1iqq/pdb
PDB entry 1iqq coloured by chain, front view.
PDB entry 1iqq coloured by chain, side view.
PDB entry 1iqq coloured by chain, top view.
Source organism: Pyrus pyrifolia
Primary publication:
Crystal structure at 1.5-A resolution of Pyrus pyrifolia pistil ribonuclease responsible for gametophytic self-incompatibility.
Matsuura T, Sakai H, Unno M, Ida K, Sato M, Sakiyama F, Norioka S
J Biol Chem 276 45261-9 (2001)
PMID: 11577107

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ribonuclease S-3 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.4 KDa
Source organism: Pyrus pyrifolia
UniProt:
  • Canonical: O80323 (Residues: 23-222; Coverage: 100%)
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, XYP, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P21
Unit cell:
a: 45.65Å b: 52.59Å c: 47.57Å
α: 90° β: 106.45° γ: 90°
R-values:
R R work R free
0.172 0.172 0.202

Quick links

Citations

6 review citations

Self-incompatibility in plants.
Takayama et al. (2005)
5 more

1 mention without citation

Evolutionary patterns at the RNase based gametophytic self - incompatibility system in two divergent Rosaceae groups (Maloideae and Prunus).
Vieira et al. (2010)