› 1iqu

X-ray diffraction
2.2Å resolution

Crystal structure of photolyase-thymine complex

Released:
DOI: 10.2210/pdb1iqu/pdb
PDB entry 1iqu coloured by chain, front view.
PDB entry 1iqu coloured by chain, side view.
PDB entry 1iqu coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of thermostable DNA photolyase: pyrimidine-dimer recognition mechanism.
Komori H, Masui R, Kuramitsu S, Yokoyama S, Shibata T, Inoue Y, Miki K
Proc Natl Acad Sci U S A 98 13560-5 (2001)
PMID: 11707580

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyribodipyrimidine photo-lyase Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.97 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61497 (Residues: 1-420; Coverage: 100%)
Gene names: TTHB102, phr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand PO4 1 x PO4
Ligand TDR 1 x TDR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P6122
Unit cell:
a: 113.099Å b: 113.099Å c: 142.554Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.211 0.243
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The cryptochromes: blue light photoreceptors in plants and animals.
Chaves et al. (2011)
10 more

1 mention without citation

Crystal structure of thermostable DNA photolyase: pyrimidine-dimer recognition mechanism.
Komori et al. (2001)