› 1isn

X-ray diffraction
2.9Å resolution

Crystal structure of merlin FERM domain

Released:
DOI: 10.2210/pdb1isn/pdb
PDB entry 1isn coloured by chain, front view.
PDB entry 1isn coloured by chain, side view.
PDB entry 1isn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for neurofibromatosis type 2. Crystal structure of the merlin FERM domain.
Shimizu T, Seto A, Maita N, Hamada K, Tsukita S, Tsukita S, Hakoshima T
J Biol Chem 277 10332-6 (2002)
PMID: 11756419

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Merlin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 38.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P46662 (Residues: 18-340; Coverage: 54%)
Gene names: Nf-2, Nf2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 67.408Å b: 67.408Å c: 179.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.229 0.284
Expression system: Escherichia coli

Quick links

Citations

25 review citations

ERM proteins and merlin: integrators at the cell cortex.
Bretscher et al. (2002)
24 more

9 mentions without citation

Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus.
Li et al. (2010)
8 more