› 1iud

X-ray diffraction
2.7Å resolution

MALTODEXTRIN-BINDING PROTEIN INSERTION/DELETION MUTANT WITH AN INSERTED B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS

Released:
DOI: 10.2210/pdb1iud/pdb
PDB entry 1iud coloured by chain, front view.
PDB entry 1iud coloured by chain, side view.
PDB entry 1iud coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a recombinant form of the maltodextrin-binding protein carrying an inserted sequence of a B-cell epitope from the preS2 region of hepatitis B virus.
Saul FA, Vulliez-le Normand B, Lema F, Bentley GA
Proteins 27 1-8 (1997)
PMID: 9037707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 41.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-159, 160-162, 163-165, 166-167, 168-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: C2221
Unit cell:
a: 61.1Å b: 97.4Å c: 137.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.281
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.
Moon et al. (2010)
4 other articles

1 mention without citation

CD4 T cell epitope specificity determines follicular versus non-follicular helper differentiation in the polyclonal response to influenza infection or vaccination.
Knowlden et al. (2016)