› 1iuo

X-ray diffraction
2Å resolution

meta-Cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) S103A mutant complexed with acetates

Released:
DOI: 10.2210/pdb1iuo/pdb
PDB entry 1iuo coloured by chain, front view.
PDB entry 1iuo coloured by chain, side view.
PDB entry 1iuo coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products.
Fushinobu S, Saku T, Hidaka M, Jun SY, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T
Protein Sci 11 2184-95 (2002)
PMID: 12192074

Function and Biology Details

Reaction catalysed: 
2-hydroxymuconate-6-semialdehyde + H(2)O = formate + 2-oxopent-4-enoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 31.51 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P96965 (Residues: 1-282; Coverage: 100%)
Gene name: cumD
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 76.543Å b: 116.619Å c: 78.037Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biodegradation of aromatic compounds: an overview of meta-fission product hydrolases.
Khajamohiddin et al. (2008)
19 other articles

1 mention without citation

Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products.
Fushinobu et al. (2002)