› 1ixh

X-ray diffraction
0.98Å resolution

PHOSPHATE-BINDING PROTEIN (PBP) COMPLEXED WITH PHOSPHATE

Released:
DOI: 10.2210/pdb1ixh/pdb
PDB entry 1ixh coloured by chain, front view.
PDB entry 1ixh coloured by chain, side view.
PDB entry 1ixh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes.
Wang Z, Luecke H, Yao N, Quiocho FA
Nat Struct Biol 4 519-22 (1997)
PMID: 9228942

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG82 (Residues: 26-346; Coverage: 100%)
Gene names: JW3706, b3728, phoS, pstS
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 41.506Å b: 63.379Å c: 122.373Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 not available 0.141
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Artificial receptors for the recognition of phosphorylated molecules.
Hargrove et al. (2011)
8 more

33 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
32 more