› 1ixk

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of Methyltransferase Homolog Protein from Pyrococcus Horikoshii

Released:
DOI: 10.2210/pdb1ixk/pdb
PDB entry 1ixk coloured by chain, front view.
PDB entry 1ixk coloured by chain, side view.
PDB entry 1ixk coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of human p120 homologue protein PH1374 from Pyrococcus horikoshii.
Ishikawa I, Sakai N, Tamura T, Yao M, Watanabe N, Tanaka I
Proteins 54 814-6 (2004)
PMID: 14997580

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAM-dependent MTase RsmB/NOP-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.26 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O50082 (Residues: 1-315; Coverage: 100%)
Gene name: PH1374
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 63.554Å b: 41.513Å c: 71.065Å
α: 90° β: 114.18° γ: 90°
R-values:
R R work R free
0.199 0.199 0.235
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu et al. (2017)
4 more

4 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
3 more