› 1iyb

X-ray diffraction
1.5Å resolution

Crystal Structure of the Nicotiana glutinosa Ribonuclease NW

Released:
DOI: 10.2210/pdb1iyb/pdb
PDB entry 1iyb coloured by chain, front view.
PDB entry 1iyb coloured by chain, side view.
PDB entry 1iyb coloured by chain, top view.
Source organism: Nicotiana glutinosa
Primary publication:
Guanine binding site of the Nicotiana glutinosa ribonuclease NW revealed by X-ray crystallography.
Kawano S, Kakuta Y, Kimura M
Biochemistry 41 15195-202 (2002)
PMID: 12484757

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease NW Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.13 KDa
Source organism: Nicotiana glutinosa
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q7XZV5 (Residues: 26-229; Coverage: 100%)
Gene name: ngr1
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

1 bound ligand:
Ligand 5GP 2 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 44.8Å b: 94.3Å c: 98.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.23
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

T2 Family ribonucleases: ancient enzymes with diverse roles.
Luhtala et al. (2010)
3 other articles

2 mentions without citation

A new family of membrane electron transporters and its substrates, including a new cell envelope peroxiredoxin, reveal a broadened reductive capacity of the oxidative bacterial cell envelope.
Cho et al. (2012)
1 more