› 1iz0

X-ray diffraction
2.3Å resolution

Crystal Structures of the Quinone Oxidoreductase from Thermus thermophilus HB8 and Its Complex with NADPH

Released:
DOI: 10.2210/pdb1iz0/pdb
PDB entry 1iz0 coloured by chain, front view.
PDB entry 1iz0 coloured by chain, side view.
PDB entry 1iz0 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of the quinone oxidoreductase from Thermus thermophilus HB8 and its complex with NADPH: implication for NADPH and substrate recognition.
Shimomura Y, Kakuta Y, Fukuyama K
J Bacteriol 185 4211-8 (2003)
PMID: 12837796

Function and Biology Details

Reaction catalysed: 
NADPH + 2 quinone = NADP(+) + 2 semiquinone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185246 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl reductase (ER) domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 32.36 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L3C8 (Residues: 1-302; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6122
Unit cell:
a: 77.687Å b: 77.687Å c: 236.629Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.249
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The type I fatty acid and polyketide synthases: a tale of two megasynthases.
Smith et al. (2007)
2 more

3 mentions without citation

SBSPKS: structure based sequence analysis of polyketide synthases.
Anand et al. (2010)
2 more