› 1izc

X-ray diffraction
1.7Å resolution

Crystal Structure Analysis of Macrophomate synthase

Released:
DOI: 10.2210/pdb1izc/pdb
PDB entry 1izc coloured by chain, front view.
PDB entry 1izc coloured by chain, side view.
PDB entry 1izc coloured by chain, top view.
Source organism: Macrophoma commelinae
Primary publication:
Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase.
Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I
Nature 422 185-9 (2003)
PMID: 12634789

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HpcH/HpaI aldolase/citrate lyase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 36.27 KDa
Source organism: Macrophoma commelinae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UVD4 (Residues: 1-339; Coverage: 100%)
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand PYR 1 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P6322
Unit cell:
a: 106.678Å b: 106.678Å c: 120.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Dearomatization strategies in the synthesis of complex natural products.
Roche et al. (2011)
18 more

2 mentions without citation

Natural [4 + 2]-Cyclases.
Jeon et al. (2017)
1 more