› 1j04

X-ray diffraction
2.6Å resolution

Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro

Released:
DOI: 10.2210/pdb1j04/pdb
PDB entry 1j04 coloured by chain, front view.
PDB entry 1j04 coloured by chain, side view.
PDB entry 1j04 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural implications of a G170R mutation of alanine:glyoxylate aminotransferase that is associated with peroxisome-to-mitochondrion mistargeting.
Djordjevic S, Zhang X, Bartlam M, Ye S, Rao Z, Danpure CJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 233-6 (2010)
PMID: 20208150

Function and Biology Details

Reactions catalysed: 
L-alanine + glyoxylate = pyruvate + glycine
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149288 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--glyoxylate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21549 (Residues: 1-392; Coverage: 100%)
Gene names: AGT1, AGXT, SPAT
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AOA 1 x AOA
Ligand GOL 7 x GOL
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 89.491Å b: 89.491Å c: 142.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.273
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Four of the most common mutations in primary hyperoxaluria type 1 unmask the cryptic mitochondrial targeting sequence of alanine:glyoxylate aminotransferase encoded by the polymorphic minor allele.
Fargue et al. (2013)
7 more

3 mentions without citation

A bioinformatic and mechanistic study elicits the antifibrotic effect of ursolic acid through the attenuation of oxidative stress with the involvement of ERK, PI3K/Akt, and p38 MAPK signaling pathways in human hepatic stellate cells and rat liver.
He et al. (2015)
2 more