› 1j47

Solution NMR

3D Solution NMR Structure of the M9I Mutant of the HMG-Box Domain of the Human Male Sex Determining Factor SRY Complexed to DNA

Released:
DOI: 10.2210/pdb1j47/pdb
PDB entry 1j47 coloured by chain, front view.
PDB entry 1j47 coloured by chain, side view.
PDB entry 1j47 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for SRY-dependent 46-X,Y sex reversal: modulation of DNA bending by a naturally occurring point mutation.
Murphy EC, Zhurkin VB, Louis JM, Cornilescu G, Clore GM
J Mol Biol 312 481-99 (2001)
PMID: 11563911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113986 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Sex-determining region Y protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 10.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05066 (Residues: 57-140; Coverage: 41%)
Gene names: SRY, TDF
Sequence domains: HMG (high mobility group) box
Structure domains: High mobility group box domain
5'-D(*CP*CP*TP*GP*CP*AP*CP*AP*AP*AP*CP*AP*CP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*GP*TP*GP*TP*TP*TP*GP*TP*GP*CP*AP*GP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.38 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
11 more

3 mentions without citation

Interpreting protein/DNA interactions: distinguishing specific from non-specific and electrostatic from non-electrostatic components.
Privalov et al. (2011)
2 more