› 1j4g

X-ray diffraction
2Å resolution

crystal structure analysis of the trichosanthin delta C7

Released:
DOI: 10.2210/pdb1j4g/pdb
PDB entry 1j4g coloured by chain, front view.
PDB entry 1j4g coloured by chain, side view.
PDB entry 1j4g coloured by chain, top view.
Source organism: Trichosanthes kirilowii
Entry authors: Ding Y, Too HM, Wang ZL, Liu YW, Dong YC, Shaw PC, Rao ZH

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein alpha-trichosanthin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 27.3 KDa
Source organism: Trichosanthes kirilowii
Expression system: Escherichia coli
UniProt:
  • Canonical: P09989 (Residues: 24-270; Coverage: 93%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 71.56Å b: 74.4Å c: 87.56Å
α: 90° β: 96.96° γ: 90°
R-values:
R R work R free
0.174 0.174 0.235
Expression system: Escherichia coli

Quick links