› 1j6t

Solution NMR

COMPLEX OF ENZYME IIAMTL AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb1j6t/pdb
PDB entry 1j6t coloured by chain, ensemble of 3 models, front view.
PDB entry 1j6t coloured by chain, ensemble of 3 models, side view.
PDB entry 1j6t coloured by chain, ensemble of 3 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of the phosphoryl transfer complex between the cytoplasmic A domain of the mannitol transporter IIMannitol and HPr of the Escherichia coli phosphotransferase system.
Cornilescu G, Lee BR, Cornilescu CC, Wang G, Peterkofsky A, Clore GM
J Biol Chem 277 42289-98 (2002)
PMID: 12202490

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132713 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system mannitol-specific EIICBA component Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00550 (Residues: 491-637; Coverage: 23%)
Gene names: JW3573, b3599, mtlA
Sequence domains: Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
Structure domains: Mannitol-specific EII; Chain A
Phosphocarrier protein HPr Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CONJOINED RIGID BODY/TORSION ANGLE DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

6 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
5 more

7 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
6 more