› 1j7i

X-ray diffraction
3.2Å resolution

Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa Apoenzyme

Released:
DOI: 10.2210/pdb1j7i/pdb
PDB entry 1j7i coloured by chain, front view.
PDB entry 1j7i coloured by chain, side view.
PDB entry 1j7i coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural analyses of nucleotide binding to an aminoglycoside phosphotransferase.
Burk DL, Hon WC, Leung AK, Berghuis AM
Biochemistry 40 8756-64 (2001)
PMID: 11467935

Function and Biology Details

Reaction catalysed: 
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 31.01 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 1-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 55.662Å b: 55.662Å c: 185.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Molecular understanding of aminoglycoside action and resistance.
Jana et al. (2006)
8 more

4 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
3 more