› 1j7j

X-ray diffraction
2.3Å resolution

Crystal Structure of the HPRT from Salmonella typhimurium

Released:
DOI: 10.2210/pdb1j7j/pdb
PDB entry 1j7j coloured by chain, front view.
PDB entry 1j7j coloured by chain, side view.
PDB entry 1j7j coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Entry authors: Lee CC, Focia PJ, Spraggon G, Eakin AE

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: O33799 (Residues: 1-178; Coverage: 100%)
Gene names: STM0170, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P3121
Unit cell:
a: 83.897Å b: 83.897Å c: 168.752Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.237
Expression system: Escherichia coli

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