› 1j8f

X-ray diffraction
1.7Å resolution

HUMAN SIRT2 HISTONE DEACETYLASE

Released:
DOI: 10.2210/pdb1j8f/pdb
PDB entry 1j8f coloured by chain, front view.
PDB entry 1j8f coloured by chain, side view.
PDB entry 1j8f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the histone deacetylase SIRT2.
Finnin MS, Donigian JR, Pavletich NP
Nat Struct Biol 8 621-5 (2001)
PMID: 11427894

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase sirtuin-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 323 amino acids
Theoretical weight: 36.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXJ6 (Residues: 34-356; Coverage: 83%)
Gene names: SIR2L, SIR2L2, SIRT2
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 78.943Å b: 119.07Å c: 218.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

78 review citations

The Sir2 family of protein deacetylases.
Blander et al. (2004)
77 more

42 mentions without citation

Sirtuins: Sir2-related NAD-dependent protein deacetylases.
North et al. (2004)
41 more